The first week of development
Ovarian cycle: At puberty, in every month ovary shows a cyclical change under the influence of gonadotropin-releasing hormone, from the hypothalamus. GRH acts on the anterior pituitary to release FSH and LH. The changes in the ovary every month known as the ovarian cycle.
FSH: follicle growth and maturation, secretion of estrogen, proliferative stage of menstruation.
LH causes ovulation, stimulate the production of progesterone.
Ovulation: shedding of the secondary oocyte (graffian follicle of ovum) with surrounding granulosa cells by rupturing the ovarian wall into the abdominal cavity is known as ovulation.
Time estimation :15+_or – 2 days
Cause: LH Surge
Clinically: ovulation can be felt by ovulation pain and increased body temperature 1 F
After ovulation, granulosa cells remain in the wall of the ruptured follicle together with cells from theca interna, become vascularized and develop and a yellowish pigment called Corpus luteum.
Function: secrete progesterone and maintain the secretory stage of menstruation.
- Corpus luteum of menstruation (remain 9 days)
- Corpus luteum of pregnancy (remain up to 4 months)
Fertilization: It is the process by which mature male gamete fused with the mature female gamete to form a zygote in the ampullary region of the uterine tube. ( Because it is the widest part of the tube and closed to the ovary)
Prerequisite for fertilization
- Capacitation: It is a period of conditioning in the female reproductive tract. Where glycoprotein coat and seminal plasma protein are removed from the plasma membrane that overlies the acrosomal region of spermatozoa. This period lasts for 7 hrs.
- Acrosomal reaction: occurs after binding to the Zona pellucida and is induced by Zona protein. This reaction culminates in the release of enzymes needed to penetrate the Zona pellucida including acrosine and traipsing like substances
Phases of fertilization
Results of fertilization
- Formation of zygote
- Initiation of cleavage
- Resumption of 2nd meiotic division
- Chromosomal sex can be determined
- Metabolic activation of the egg.
Cleavage: It is the process by which one cell zygote undergoes a series of mitotic divisions.
Blastomere: The cells which become smaller with each cleavage division are known as blastomere.
Morula: 16 cell stage is known as a morula.
Blastocyst: When fluid begins to appear in the intercellular spaces of the inner cell mass, they confluent to form a single cavity, blastocele, at this time the embryo is called the blastocyst. The Zona pellucida disappears.
Inner cell mass (embryoblast)
Outer cell mass(trophoblast)??become the placenta
Implantation: it is the process by which blastocyst embedded on the mucus membrane of the uterine wall.
Site: It occurs on the anterior or posterior wall of the uterus near the fundus. On the 6th day of development.
- The lower end of the uterus(placenta previa)
- Uterine tube (tubal pregnancy)
- Ovary (ovarian pregnancy)
- Abdominal cavity
Note: when implantation occurs other than normal site is known as abnormal implantation.
Ectopic pregnancy other than the uterus
- Why 2nd week is called the week of two's?
- 2 cavity
- Amniotic cavity
- Chorionic cavity
- 2 yolk sac
- Primitive yolk sac
- Secondary (definitive) yolk sac
Bilaminar germ disc: Epiblast and hypoblast together form bilaminar germ disc.
Decidua: Endometrium of the uterus at the time of pregnancy is known as decidua.
Decidual reaction: During pregnancy, cells of the endometrium become polyhedral, loaded with glycogen and lipid, intercellular space filled with extravasate and the tissue is redementus . These changes are known as a decidual reaction.
Events of 3rd week:
- Notified formation
Gastrulation: The process that establishes all three germ layers (ectoderm, mesoderm, and endoderm) in the embryo is called gastrulation.
It begins with the formation of the premative streak on the surface of the epiblast. The premative streak is a narrow groove with a slightly raised area on either side. Cells of the epiblast migrate towards the premative streak and pass through it, this inward movement is known as invagination. Some invagination cells displace the hypoblast and create the embryonic endoderm. Cells come to lie between the epiblast and endoderm to form mesoderm. Cells in the epiblast become ectoderm.
- Surface ectoderm
- Ectodermal placode
Surface ectoderm: Epidermis of the skin including hairs, nails, sebaceous glands, sweat glands, lining epithelium of cheek, gum, mouth, nasal cavity, paranasal air sinuses, salivary glands, the lower part of the anal canal and urethra.
- Neural tube:
- Central nervous system
- Brain and spinal cord
- Neural crest
- Bones of faces and skull
- Laptomeninges(arachnoid+pia mater)
- Ganglia( sensory, autonomic, dorsal root)
- Adrenal/suprarenal gland(medulla)
- C-cell of the thyroid gland
Otic placode-internal ear
Neurulation: The conversion of the neural plate into a neural tube by folding is known as neurulation.
Due to the influence of notochord, overlying ectoderm thickened to form the neural plate. The lateral edges of the neural plate become more elevated to form neural folds and neural groove forms in the mid-region. Gradually neural folds fused and neural tube formed. Cephalic opening (24th day) of the neural tube is known as cranial neuropore. And caudal opening (27th day) is known as caudal neuropore. Finally the neuropore is closed.
The fate of the neural tube: The cranial portion becomes the brain and caudal becomes the spinal cord.
- What is anencephaly?
Ans. Exposure of brain tissue due to the absence of cranial vault. It is due to the failure of closure of cranial neuropore.
Spina – bifida: If caudal neuropore doesn’t close then spina bifida develops.
Neural crest: cells of the lateral border or crest of the neuroectoderm is known as neural crest.
Process of notochord formation:
- Formation of notochord or head process
- Formation of neurouterine canal
- Formation of notochord plate
- Formation of the definite notochord.
- Paraxial mesoderm-somite -vertebral column
- Intermediate mesoderm-genito-urinary system
- Lateral plate mesoderm
- Somatopleuric(parietal layer of the serous membrane )
- Splanchnopleuric (heart and blood vessels, viscera)
Somite: segmental blocks of paraxial mesoderm are called somite. There are 42-44 pairs of somites.
Divided into three parts;
- Sclerotome(vertebral column)
- Dermatome( dermis of back and subcutaneous tissue)
Each somite has its own sclerotome, myotome, and dermatome.
Derivatives of mesoderm
- All connective tissue and sclerosis tissue.
- Teeth except for enamel
- All muscles of the body( except muscles of iris and erector pullorum)
- CVS and lymphatic system
- Urogenital system
- Suprarenal cortex and glands
- The mesothelial lining of pericardial, pleural, and peritoneal cavities.
- Yolk sac
- Connecting stalk
- Vitello intestinal duct
- Umbilical cord
Morphology of placenta:
Surface: fetal surface – smooth, covered by the amnion, and present the attachment of the umbilical cord.
Maternal surface: rough and irregular, present 15 to 25 cotyledons covered by decidua basalis.
Structure: chorionic plate on the fetal side, basal plate on the maternal side. Between them lies stem villi. Between the stem, villi lie maternal blood.
- Primary mesoderm continuing branches of umbilical vessels
Urachus: when the lumen of the allantois is obliterated a thick fibrous cord remains and connects the apex of the bladder with umbilicus is known as urachus.
- Decidua basalis
- The outer layer of syncytiotrophoblast
- The outer shell of cytotrophoblast
- The inner layer of syncytiotrophoblast
Development of placenta:
Maternal side- decidua basalis
Fetal side- chorionic frondosum
Decidua: Endometrium of the uterus at the time of pregnancy is called decidua.
Type: 3 types
- Decidua basalis
- Decidua pareitalis
- Decidua capsularis
Chorione: 1. Chorionic leabe- smooth
- Chorionic frondosum
Note: human placenta – haemochorial type
- Exchanges of gases(O2, Co2, co)
- Exchange of nutrients and electrolyte- amino acid, free fatty acid, carbohydrate, vitamins
- IgG- transmission of maternal antibody.
- Hormone productions – progesterone,estrogen (estriol) ,human chorionic gonadotropin ( HCG- releases from Corpus luteum), placental lactogen.
- Before 4th month
- Connective tissue
- After 4th month
Allantois: As a result of cephalocaudal and lateral folding of the embryo, a portion of the endoderm lined cavity remain outside the embryo known as the allantois